乳酸脱水素酵素の反応機構

乳酸脱水素酵素の反応機構

by 孝昌 野中 -

\put(-950,1500){\tetramethylene{1==H$\bf_3$C;4==OH}{2D==O;3D==O}\ \raisebox{7mm}{\reactrarrow{0pt}{3.5cm}{\sffamily\bfseries NADH,\ H$^+$}{乳酸脱水素酵素}}\spc\tetramethylene{1==H$\bf_3$C;4==OH}{2SA==OH;2SB==H;3D==O}\put(-2700,-200){$\text{ピルビン酸}\hspace{5.75cm}\text{L-乳酸}$}\put(-3030,-270){\tikz{\draw[line width=0.8pt](0,0) rectangle (11,3)}}}\sixheterov[]{4==\downnobond{O}{\adddash(40,80)(0,-1){200}};1s==\trimethylenei{}{1==(yl);2D==O}}{1Sd==\put(-70,0){\upnobond{N}{\put(40,0){\exosinglebond{0}}}};1Su==H;5==O}[abc]\WavyAsSubstX(-570,1000)(-370,1000)\WavyAsSubstX(-80,530)(-80,730)\put(-695,170){\tikz{\draw[line width=0.8pt,dashed] (0,0) arc [start angle=35, delta angle=110,radius=0.5];\node at (-0.4,0) {-};}}\put(-810,150){\tikz{\draw[line width=0.8pt](0,0) rectangle (2.95,3.26) node[right=1.6cm,pos=0.5]{アミノ酸残基 168 番}}}\put(-525,-550){\xFiveCycle(0,0){0}[b]{1s==\exosinglebond{145};4s==\exosinglebond{0};1==\sffamily\bfseries N;4==\sffamily\bfseries N;3s==\trimethylene{3==His 195;}{1==(yl)}}[]\put(-140,-210){\sffamily\bfseries H}\put(60,480){\sffamily\bfseries H}\put(-15,30){\tikz{\draw[line width=0.8pt,dashed] (0,0) arc [start angle=110, delta angle=110,radius=0.5];\node at (-0.1,-0.4) {+};}}\put(450,-200){\tikz{\draw[line width=0.8pt] (0,0) arc [start angle=130, delta angle=90,radius=2]}}}\put(-1850,-1065){\decaheterovb[]{1==N;2==H;8==NH;9==H$\bf_2$N}{1==H;7==Arg 109}[bcdefg]\put(-570,470){\tikz{\draw[line width=0.8pt,dashed] (0,0) arc [start angle=35, delta angle=110,radius=0.5];\node at (-0.4,0) {+};}}\put(-1000,880){\tikz{\draw[line width=0.8pt] (0,0) arc [start angle=240, delta angle=90,radius=2.3]}}}\put(-1270,-1785){\sixheterov[]{3==O;6==H$\bf_3$C}{1D==O;2==O}[cde]\adddash(-370,880)(7,7){200}\adddash(-420,880)(-7,7){200}\adddash(-20,610)(7,-4){200}\adddash(-200,300)(7,-4){200}\put(-200,380){\tikz{\draw[line width=0.8pt,dashed] (0,0) arc [start angle=100, delta angle=100,radius=0.5];\node at (-0.1,-0.4) {-};}}\put(-390,720){\tikz{\draw[line width=0.8pt,-latex] (0,0) arc [start angle=270, delta angle=125,radius=0.5]}}}\put(-650,-1950){\decaheterov[]{4==\downnobond{N}{H};1==H;5==N;6==H;8==H;{{10}}==N}{3==Arg 171;5==H}[abgh]\put(-815,315){\tikz{\draw[line width=0.8pt,dashed] (0,0) arc [start angle=270, delta angle=110,radius=0.5];\node at (0.2,0.3) {+};}}\put(-300,-150){\tikz{\draw[line width=0.8pt] (0,0) arc [start angle=130, delta angle=90,radius=2]}}}\put(-1500,-2300){\sixheterov[be]{4==\upnobond{N}{..}}{1Sa==H;1Sb==H;4==R;6==H$\bf_2$NOC}[]\put(-405,295){\tikz{\draw[line width=0.8pt,-latex] (0,0) .. controls(0.08,0.2) and (0.25,0.2) .. (0.39,-0.18)}}\put(-375,440){\tikz{\draw[line width=0.8pt,-latex] (0,0) .. controls(-0.4,0.01) and (-0.3,0.35) .. (-0.2,0.55)}}\put(-1000,100){\tikz{\draw[line width=0.8pt](3,0)node[draw,rectangle,rounded corners=0pt] {\sffamily\bfseries NADH}}}\put(-370,700){\tikz{\draw[line width=0.8pt,-latex] (0,0) .. controls(0.8,0.2) and (0.75,1.2) .. (0.7,1.6)}}}\put(-1300,-2500){乳酸脱水素酵素の活性中心（模式図）}